Molecular cloning and biological activity of alpha-, beta-, and gamma-megaspermin, three elicitins secreted by Phytophthora megasperma H20.
Identifieur interne : 002438 ( Main/Exploration ); précédent : 002437; suivant : 002439Molecular cloning and biological activity of alpha-, beta-, and gamma-megaspermin, three elicitins secreted by Phytophthora megasperma H20.
Auteurs : Fabienne Baillieul [France] ; Patrice De Ruffray ; Serge KauffmannSource :
- Plant physiology [ 0032-0889 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN complémentaire (composition chimique), ADN complémentaire (génétique), Analyse de séquence d'ADN (MeSH), Analyse de séquence de protéine (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Extraits de plantes (métabolisme), Isoformes de protéines (génétique), Isoformes de protéines (métabolisme), Phylogenèse (MeSH), Phytophthora (génétique), Phytophthora (métabolisme), Protéines (MeSH), Protéines d'algue (génétique), Protéines d'algue (métabolisme), Protéines végétales (métabolisme), Scopolétine (métabolisme), Sesquiterpènes (MeSH), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Tabac (génétique), Tabac (microbiologie), Terpènes (MeSH).
- MESH :
- composition chimique : ADN complémentaire.
- génétique : ADN complémentaire, Isoformes de protéines, Phytophthora, Protéines d'algue, Tabac.
- microbiologie : Tabac.
- métabolisme : Extraits de plantes, Isoformes de protéines, Phytophthora, Protéines d'algue, Protéines végétales, Scopolétine.
- Analyse de séquence d'ADN, Analyse de séquence de protéine, Clonage moléculaire, Données de séquences moléculaires, Phylogenèse, Protéines, Sesquiterpènes, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique, Terpènes.
English descriptors
- KwdEn :
- Algal Proteins (genetics), Algal Proteins (metabolism), Amino Acid Sequence (MeSH), Base Sequence (MeSH), Cloning, Molecular (MeSH), DNA, Complementary (chemistry), DNA, Complementary (genetics), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Phytophthora (genetics), Phytophthora (metabolism), Plant Extracts (metabolism), Plant Proteins (metabolism), Protein Isoforms (genetics), Protein Isoforms (metabolism), Proteins (MeSH), Scopoletin (metabolism), Sequence Analysis, DNA (MeSH), Sequence Analysis, Protein (MeSH), Sequence Homology, Amino Acid (MeSH), Sesquiterpenes (MeSH), Terpenes (MeSH), Tobacco (genetics), Tobacco (microbiology).
- MESH :
- chemical , chemistry : DNA, Complementary.
- chemical , genetics : Algal Proteins, DNA, Complementary, Protein Isoforms.
- chemical , metabolism : Algal Proteins, Plant Extracts, Plant Proteins, Protein Isoforms, Scopoletin.
- genetics : Phytophthora, Tobacco.
- metabolism : Phytophthora.
- microbiology : Tobacco.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Sequence Data, Phylogeny, Proteins, Sequence Analysis, DNA, Sequence Analysis, Protein, Sequence Homology, Amino Acid, Sesquiterpenes, Terpenes.
Abstract
We report on the molecular cloning of the Phytophthora megasperma H20 (PmH20) glycoprotein shown previously as an inducer of the hypersensitive response, of localized acquired resistance and of systemic acquired resistance in tobacco (Nicotiana tabacum), and of the PmH20 alpha- and beta-megaspermin, two elicitins of class I-A and I-B, respectively. The structure of the glycoprotein shows a signal peptide of 20 amino acids followed by the typical elicitin 98-amino acid-long domain and a 77-amino acid-long C-terminal domain carrying an O-glycosylated moiety. The molecular mass deduced from the translated cDNA sequence is 14,920 and 18,676 D as determined by mass spectrometry. This structure together with multiple sequence alignments and phylogenetic analyses indicate that the glycoprotein belongs to class III elicitins. It is the first class III elicitin protein characterized, which we named gamma-megaspermin. We compared the biological activity of the three PmH20 elicitins when applied to tobacco cv Samsun NN plants. Although alpha- and gamma-megaspermin were similarly active, beta-megaspermin was the most active in inducing the hypersensitive response and localized acquired resistance, which was assessed by measuring the levels of acidic and basic pathogenesis-related proteins and of the antioxidant phytoalexin scopoletin. The three elicitins induced similar levels of systemic acquired resistance measured as the expression of acidic PR proteins and is increased resistance to challenge tobacco mosaic virus infection.
DOI: 10.1104/pp.012658
PubMed: 12529524
PubMed Central: PMC166796
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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nucléotidique</term><term>Terpènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report on the molecular cloning of the Phytophthora megasperma H20 (PmH20) glycoprotein shown previously as an inducer of the hypersensitive response, of localized acquired resistance and of systemic acquired resistance in tobacco (Nicotiana tabacum), and of the PmH20 alpha- and beta-megaspermin, two elicitins of class I-A and I-B, respectively. The structure of the glycoprotein shows a signal peptide of 20 amino acids followed by the typical elicitin 98-amino acid-long domain and a 77-amino acid-long C-terminal domain carrying an O-glycosylated moiety. The molecular mass deduced from the translated cDNA sequence is 14,920 and 18,676 D as determined by mass spectrometry. This structure together with multiple sequence alignments and phylogenetic analyses indicate that the glycoprotein belongs to class III elicitins. It is the first class III elicitin protein characterized, which we named gamma-megaspermin. We compared the biological activity of the three PmH20 elicitins when applied to tobacco cv Samsun NN plants. Although alpha- and gamma-megaspermin were similarly active, beta-megaspermin was the most active in inducing the hypersensitive response and localized acquired resistance, which was assessed by measuring the levels of acidic and basic pathogenesis-related proteins and of the antioxidant phytoalexin scopoletin. The three elicitins induced similar levels of systemic acquired resistance measured as the expression of acidic PR proteins and is increased resistance to challenge tobacco mosaic virus infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12529524</PMID><DateCompleted><Year>2003</Year><Month>05</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>131</Volume><Issue>1</Issue><PubDate><Year>2003</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning and biological activity of alpha-, beta-, and gamma-megaspermin, three elicitins secreted by Phytophthora megasperma H20.</ArticleTitle><Pagination><MedlinePgn>155-66</MedlinePgn></Pagination><Abstract><AbstractText>We report on the molecular cloning of the Phytophthora megasperma H20 (PmH20) glycoprotein shown previously as an inducer of the hypersensitive response, of localized acquired resistance and of systemic acquired resistance in tobacco (Nicotiana tabacum), and of the PmH20 alpha- and beta-megaspermin, two elicitins of class I-A and I-B, respectively. The structure of the glycoprotein shows a signal peptide of 20 amino acids followed by the typical elicitin 98-amino acid-long domain and a 77-amino acid-long C-terminal domain carrying an O-glycosylated moiety. The molecular mass deduced from the translated cDNA sequence is 14,920 and 18,676 D as determined by mass spectrometry. This structure together with multiple sequence alignments and phylogenetic analyses indicate that the glycoprotein belongs to class III elicitins. It is the first class III elicitin protein characterized, which we named gamma-megaspermin. We compared the biological activity of the three PmH20 elicitins when applied to tobacco cv Samsun NN plants. Although alpha- and gamma-megaspermin were similarly active, beta-megaspermin was the most active in inducing the hypersensitive response and localized acquired resistance, which was assessed by measuring the levels of acidic and basic pathogenesis-related proteins and of the antioxidant phytoalexin scopoletin. The three elicitins induced similar levels of systemic acquired resistance measured as the expression of acidic PR proteins and is increased resistance to challenge tobacco mosaic virus infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Baillieul</LastName><ForeName>Fabienne</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie et Physiologie Végétales, Unité de Formation et de Recherche des Sciences, Université de Reims, Boite Postale 1039, 51687 Reims, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Ruffray</LastName><ForeName>Patrice</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Kauffmann</LastName><ForeName>Serge</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020033" MajorTopicYN="N">Protein Isoforms</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012603" MajorTopicYN="N">Scopoletin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020539" MajorTopicYN="N">Sequence Analysis, Protein</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012717" MajorTopicYN="N">Sesquiterpenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013729" MajorTopicYN="N">Terpenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" 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Est</li></region><settlement><li>Reims</li></settlement></list><tree><noCountry><name sortKey="De Ruffray, Patrice" sort="De Ruffray, Patrice" uniqKey="De Ruffray P" first="Patrice" last="De Ruffray">Patrice De Ruffray</name><name sortKey="Kauffmann, Serge" sort="Kauffmann, Serge" uniqKey="Kauffmann S" first="Serge" last="Kauffmann">Serge Kauffmann</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Baillieul, Fabienne" sort="Baillieul, Fabienne" uniqKey="Baillieul F" first="Fabienne" last="Baillieul">Fabienne Baillieul</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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